gmuF [2018-06-22 18:35:02]

mannose-6-P-isomerase

locus: BSU05870

pI: 5.89

mw: 35.34 kDa

protein length: 315 aa

gene length: 945 bp

function: glucomannan utilization

product: mannose-6-P-isomerase

essential: no

synonyms: ydhS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]

Gene

Coordinates: 631,808 → 632,755

The protein

Paralogous protein(s)

• [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|Pmi], [protein|E26C70893C5D677C816C814558CC42F90B920087|ManA]

Structure

• [PDB|1QWR] ([protein|E26C70893C5D677C816C814558CC42F90B920087|ManA], 58% identity)

Expression and Regulation

Operons

• genes: [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
  description: [Pubmed|18177310]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]

Biological materials

Mutant

• MGNA-C194 (ydhS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2192 NBRP B. subtilis, Japan]
• BKE05870 (Δ[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGCTCTAAAAATAATGGAT, downstream forward: _UP4_CCTTAATGAATGGGGGAGTT
• BKK05870 (Δ[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGCTCTAAAAATAATGGAT, downstream forward: _UP4_CCTTAATGAATGGGGGAGTT

References

• 18177310,19447949,20817675